Human Anti-Human C5 Rcombinant Antibody (TP-092CL)
CAT#: TP-092CL
This antiboy is a recombinant humanized monoclonal IgG2/4;κ antibody produced by murine myeloma cell culture and purified by standard bioprocess technology. It contains human constant regions from human IgG2 sequences and human IgG4 sequences and murine complementarity-determining regions grafted onto the human framework light- and heavy-chain variable regions. This antibody is composed of two 448 amino acid heavy chains and two 214 amino acid light chains and has a molecular weight of approximately 148 kDa.
Tested Data
SDS-PAGE
Figure 1 Anti-Human C5 Recombinant Antibody (TP-092CL) in SDS-PAGE
SDS-PAGE analysis of TP-092CL in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
WB
Figure 2 Anti-Human C5 Recombinant Antibody (TP-092CL) in WB
Western blot analysis of TP-092CL was performed by loading Human C5 protein.
TP-092CL incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
ELISA
Figure 3 Anti-Human C5 Recombinant Antibody (TP-092CL) in ELISA
ELISA analysis of TP-092CL was performed by coating with Human C5 protein.
The secondary antibody: HRP-Anti-Human IgG (H+L)
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Specifications
- Type
- Biotech
Target
- Function
- Humanized mAb that binds complement protein C5 and inhibits its cleavage to C5a and C5b, preventing the formation of the terminal complement complex C5b–9
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Customer Review
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Michael Thompson29.Mar,23
Excellent for Complement Research
The Eculizumab has been a fantastic addition to our complement research. Its specificity and sensitivity are outstanding, providing clear and reliable results. This antibody has become essential for our experiments, improving the accuracy and reliability of our assays.
Sarah Harris22.Jun,22
Effective and Reliable
We incorporated this antibody into our complement assays, and it has been a great asset. The specificity for complement proteins is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
Brian White30.Aug,21
Consistent High-Quality Results
This antibody has proven to be consistent and reliable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for complement proteins have been particularly beneficial.
Q&As
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Is the Eculizumab antibody suitable for use in ELISA?
A: Yes, the Eculizumab antibody (TP-092CL) is suitable for use in ELISA. It has been validated for use in such assays and provides reliable detection of the target.
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What is the optimal dilution for using the Eculizumab antibody in Western blotting?
A: The optimal dilution for using the Eculizumab antibody (TP-092CL) in Western blotting is typically 1:500 to 1:2000. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.
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Can the Eculizumab antibody be used in immunofluorescence assays?
A: Yes, the Eculizumab antibody (TP-092CL) can be used in immunofluorescence assays. It provides specific binding to the target, allowing for detailed visualization of its expression in cells.
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Is the Eculizumab antibody effective in immunoprecipitation assays?
A: Yes, the Eculizumab antibody (TP-092CL) is effective in immunoprecipitation assays. It provides specific binding to the target protein, enabling its precipitation from complex mixtures for further analysis.
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What are the recommended storage conditions for the Eculizumab antibody?
A: The recommended storage condition for the Eculizumab antibody (TP-092CL) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TP-092CL, RRID: AB_3112052)
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Biosimilar Overview
Please refer to Eculizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Eculizumab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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